TGFBR2

TGFBR2 PCR Primer
Target	Primer name	Sense primer (5'->3')	Anti-sense primer (5'->3')	Product size [bp]	Annealing temperature[oC]
Exon 1	TGFBR2EX1	CGAGGAGAGGGAGAAGGCTC	CGACTGTCAAGCGCAGC	559	60
Exon 1	TGFBR2EX1_2	CATGATTGGCAGCTACGAGAG	CGACTGTCAAGCGCAGC	598	60
Exon 2	TGFBR2EX2	TTCAGGAATTCATTGGCAGG	CGTACACATACATGCAGAGAACA	426	65
Exon 3	TGFBR2EX3	AGCCTGCGAATGCTGGAGA	CCACCAGCTGAATGAGCTCCT	436	65
Exon 4	TGFBR2EX4	ATGCTCAGGCATGAACCCACT	CAGTGTCCAGAGTACAGGGCTG	991	65
Exon 5	TGFBR2EX5	AATCCTCTGCACGTGTCAGG	TGCTCGAAGCAACACATGATC	312	65
Exon 6	TGFBR2EX6	CAGGCATCTCACCATGCTCAT	CTCAATATTCCAGAATTCTCTGCCA	286	65
Exon 7	TGFBR2EX7	CAGCAGCAGGCACTCAGTCA	TATGTCACCCACTCCCTGCTG	503	65
iso	TGFBR2_iso	ATATGGGTGAAAGATCACCAGAG	CTGATGAGTACACTGCCATCG	441	60

TGFBR2 Sequence Primer
Target	Primer name	Sequence
Exon 1	TGFBR-EX1-SEQ1AF	GAGAGGGAGAAGGCTCTCG
Exon 1	TGFBR2EX1SeqF	GGACAGGAGCCGGACTC
Exon 1	TGFBR2EX1SeqR	CGCTGCTCGTCATAGAC
Exon 1	TGFBR2EX1R	CGACTGTCAAGCGCAGC
Exon 2	TGFBR2EX2F	TTCAGGAATTCATTGGCAGG
Exon 2	TGFBR2EX2R	CGTACACATACATGCAGAGAACA
Exon 3	TGFBR2EX3F	AGCCTGCGAATGCTGGAGA
Exon 3	TGFBR2EX3R	CCACCAGCTGAATGAGCTCCT
Exon 4	TGFBR2EX4F	ATGCTCAGGCATGAACCCACT
Exon 4	TGFBR2EX4SeqF	TCTGACATCAGCTCCACG
Exon 4	TGFBR2EX4SeqR	TGACTGCCACTGTCTCA
Exon 4	TGFBR2EX4R	CAGTGTCCAGAGTACAGGGCTG
Exon 5	TGFBR2EX5F	AATCCTCTGCACGTGTCAGG
Exon 5	TGFBR2EX5R	TGCTCGAAGCAACACATGATC
Exon 6	TGFBR2EX6F	CAGGCATCTCACCATGCTCAT
Exon 6	TGFBR2EX6R	CTCAATATTCCAGAATTCTCTGCCA
Exon 7	TGFBR2EX7F	CAGCAGCAGGCACTCAGTCA
Exon 7	TGFBR2EX7R	TATGTCACCCACTCCCTGCTG
TGFBR-iso	TGFBR-isof-SEQ1AF	GATCACCAGAGGGAAAAATGTC
TGFBR-iso	TGFBR-isof-R	CTGATGAGTACACTGCCATCG

管理・運営：骨系統疾患コンソーシウム

連絡先

お問い合わせは、
山田（taka0197@med.hokudai.ac.jp）宛

でお願いします。なお、臨床像のファイルが5MB以上になる場合は、CDかファイルを分割して送ってください。