でお願いします。なお、臨床像のファイルが5MB以上になる場合は、CDかファイルを分割して送ってください。
TGFBR1
| Target | Primer name | Sense primer (5'->3') | Anti-sense primer (5'->3') | Product size [bp] | Annealing temperature[oC] |
|---|---|---|---|---|---|
| Exon 1 | TGR1ex1 | cctccgagcagttacaaagg | cgccatgtttgagaaagagc | 318 | 55 |
| Exon 2 | TGR1ex2 | CAGTGCATAGAAATTGTGTGAT | TCTAAACGGAATGAGCTGGA | 475 | 60 |
| Exon 3 | TGR1ex3 | AACTGCTGTAATGAGGCTCT | TCTCACATTCTAGCAAGTTGG | 470 | 60 |
| Exon 4 | TGR1ex4 | ACTCATTAGTGCCTATCATG | GAATGCTATCAAGAGTCAAGA | 510 | 55 |
| Exon 5 | TGR1ex5 | GTGTGACTCAGGATTGAGAG | TTTCATAGACATTATTCATGAA | 324 | 55 |
| Exon 6 | TGR1ex6 | ATTGTAGGTCATGTGGGCTG | AGAGCAGGCAAGACCATGAG | 435 | 60 |
| Exon 7 | TGR1ex7 | AATGTGCAAACCAGTGTGGA | TCTCTGGCACTCGGTGACAT | 420 | 60 |
| Exon 8 | TGR1ex8 | AGCTGTAATCTCTGTTCCACA | ACATTGGTTTGACTGCTATGA | 391 | 60 |
| Target | Primer name | Sequence |
|---|---|---|
| Exon 1 | TGR1ex1F | cctccgagcagttacaaagg |
| Exon 1 | TGR1ex1R2 | cgccatgtttgagaaagagc |
| Exon 2 | TGFBR1ex2F | CAGTGCATAGAAATTGTGTGAT |
| Exon 2 | TGFBR1ex2R | TCTAAACGGAATGAGCTGGA |
| Exon 3 | TGFBR1ex3F | AACTGCTGTAATGAGGCTCT |
| Exon 3 | TGFBR1ex3R | TCTCACATTCTAGCAAGTTGG |
| Exon 4 | TGFBR1ex4F | ACTCATTAGTGCCTATCATG |
| Exon 4 | TGFBR1ex4R2 | GAATGCTATCAAGAGTCAAGA |
| Exon 5 | TGFBR1ex5F | GTGTGACTCAGGATTGAGAG |
| Exon 5 | TGR1ex5seqR | CATAGACATTATTCATGAAAATTTAAAGC |
| Exon 6 | TGFBR1ex6F | ATTGTAGGTCATGTGGGCTG |
| Exon 6 | TGFBR1ex6R | AGAGCAGGCAAGACCATGAG |
| Exon 7 | TGFBR1ex7F | AATGTGCAAACCAGTGTGGA |
| Exon 7 | TGFBR1ex7R | TCTCTGGCACTCGGTGACAT |
| Exon 8 | TGFBR1ex8F | AGCTGTAATCTCTGTTCCACA |
| Exon 8 | TGFBR1ex8R | ACATTGGTTTGACTGCTATGA |
| Exon 9 | TGFBR1ex9SeqF | TGTAGGCCTTGAGAGTA |
| Exon 9 | TGFBR1ex9SeqR | CCTCTCAGTGAGGTAGA |
連絡先
お問い合わせは、
山田（taka0197@med.hokudai.ac.jp）宛