でお願いします。なお、臨床像のファイルが5MB以上になる場合は、CDかファイルを分割して送ってください。
COMP
| Target | Primer name | Sense primer (5'->3') | Anti-sense primer (5'->3') | Product size [bp] | Annealing temperature[oC] |
|---|---|---|---|---|---|
| Exon1-3 | COMP_E1-3F/E1-3R | CCGTGCCTTGGGGATAAATA | CTGTCTCTGTGTCTCTGTCT | 847 | * |
| Exon4 | COMP_E4F/E4R | CGTGGCCATGATAGGCTCT | GAGGTGAGAGGCTCTTCGG | 366 | * |
| Exon5-6 | COMP_E5-6F/E5-6R | CATCCGAGACACTACGAGGT | TCCCGCGATCCTTTCTTCCT | 573 | * |
| Exon7-9 | COMP_E7-9F/E7-9R | AGGAAGAAAGGATCGCGGGA | CCATCTCTTGACCTCGTGAT | 700 | * |
| Exon10 | COMP_E10F/E10R | CTGAGGAGTGTGACCTTTGC | ATCCGGCTTCCAAAACCAGC | 320 | * |
| Exon11-12 | COMP_E11-12F/R | GCTGGCTCATCCTAATGAAG | CCAGAGCGTTTTGTCAAAGG | 538 | * |
| Exon13-15 | COMP_E13-15F/E13-14R | CCTTTGACAAAACGCTCTGG | GTGGCAGGATAGCGCTGCT | 739 | * |
| Exon16 | COMP_E16F/E16R | CAGGTTTGGGTTCTGGGTG | GGCTCTAAGGGCTGTAAAGG | 360 | * |
| Exon17 | COMP_E17F/E17R | ACTGTCTCTCCAGGAAGGTG | GGCTCATTCTAACTGCCCTG | 390 | * |
| Exon18-19 | COMP_i17i19F/i17i19R | CTGGCCACTGAAGCTCTGA | TCTGAGCCCTTCTCACTTCC | 463 | * |
* Unless otherwise noted, Roche Diagnostics GmbH Expand high Fidelity PCR System used denaturation at 94 oC for 30 sec annealing at 58 oC for 1 min extension at 72 oC for 1 min × 5 Cycles, and denaturation at 94 oC for 1 min annealing at 55 oC for 1 min extension at 72 oC for 1 min × 35 Cycles.
| Target | Primer name | Sequence |
|---|---|---|
| Exon1 | COMP_E1-3F | CCGTGCCTTGGGGATAAATA |
| Exon2 | COMP_i1i3F | CGAGACTAGAGGAGCGCAGT |
| Exon3 | COMP_E3SF | AGAGAGGAAGTACCCGAGAA |
| Exon4 | COMP_E4F | CGTGGCCATGATAGGCTCT |
| Exon5 | COMP_E5-6F | CATCCGAGACACTACGAGGT |
| Exon6 | COMP_E6SF | ATGGAGGGGAGAGGCAGGAT |
| Exon7 | COMP_i4i7R | GCACACTGTGGGAGAGTGTAAG |
| Exon8-9 | COMP_i7i9F2 | TTGAGGCGGGGTTGGGTG |
| Exon8-9 | COMP_E9R2 | AGGATGGTCTCCATCTCTTG |
| Exon10 | COMP_E10F | CTGAGGAGTGTGACCTTTGC |
| Exon11 | COMP_i10i11R | ATCCAACTTGCAGTTCACCC |
| Exon12 | COMP_i11e13F | TCCTCTGTCTGATTATGGACCC |
| Exon13-14 | COMP_E13-15F | CCTTTGACAAAACGCTCTGG |
| Exon13-14 | COMP_e13e14R | CCTGAAGTCGGTGAGCGT |
| Exon15 | COMP_E15SF | TTCCAGACAGTCGTGCTGGA |
| Exon16 | COMP_E16F | CAGGTTTGGGTTCTGGGTG |
| Exon17 | COMP_E17R | GGCTCATTCTAACTGCCCTG |
| Exon18-19 | COMP_i17i19F | CTGGCCACTGAAGCTCTGA |
連絡先
お問い合わせは、
山田(taka0197@med.hokudai.ac.jp)宛